Will's home tissue culture lab

[Will20013]

Hey everyone,

I've been experimenting with tissue culture a bunch these last few months in my living room. My goal is to eventually get my whole collection in culture and use the extra plants for trades or selling locally. I'll be posting pictures here of some of my cultures I've got growing as I progress!

Here are some venus flytraps that I've grown from seed in tissue culture. I'm amazed at the variation in colour that I'm seeing, and I've even got an albino plant that is barely surviving lol!

 

[Lloyd Gordon]

Albino should be fine in culture.

 

[Will20013]

Testing out liquid culture for my D.binata. You flip it every day to temporarily immerse the plants. So far it's making a nice ball of plants lol. I'm going to try this setup for my venus flytraps and see what happens!

 

[Will20013]

Sterillized a butterwort leaf and these are all the babies its made so far

 

[Lloyd Gordon]

I would have thought sterilization would kill a ping. How did you prepare it?

 

[Will20013]

So I chose a large emerging leaf, then did a 5% bleach for 10minute treatment (no tween). I thought the leaf would completely die, but some patches of the leaf stayed green. I did the same treatment to a couple other smaller species and it did not work.

 

[Lloyd Gordon]

That's interesting, I usually use 10% bleach.

 

[Will20013]

Ya I usually use 10%, but for delicate tissues I try 5%. Greater risk of contamination but for clean tissues it can work.

 

[Will20013]

The purple venus flytrap continues to streadily grow! Im wondering if its not albino, just really red. Ill try bringing it out of culture once it gets bigger to see if it survives.

 

[Les serres de Paolo Inc.]

Nice work @Will20013 , TC is so addictive!

Liquid in vitro is also cool but take a little more space (can’t stack them) and need to be flipped everyday.

One thing now I know is there’s not enough space when you TC again using already TC vessel as the quantity produced is just insane. Building our second Greenhouse and already thinking about the third one lolll

Have fun!

 

[Will20013]

Thanks @Les serres de Paolo Inc. ! Ya the liquid bioreactors are a bit of a pain to flip everyday but I am using them to rapidly grow some cultures that are slow to establish on agar. Ya it is wild how many plants can divide in a single container, you will have to build greenhouses at an exponential rate lol!!!

 

[Will20013]

I have attempted to put some sphagnum spores into tissue culture from @Smilodonichthys. It took me a few attempts to not have contamination but it seems like the spores are starting to germinate!!! I will update once they grow more, I am hoping they like the media that i have them on.

 

[Will20013]

Starting to turn more green now!!

 

[Will20013]

Update!

 

[WillyCKH]

Amazing, congrats! I tried a few different sterilizations on the spores but didn't get good results.