Rescue of Nepenthes hamata…in a funny way…
- Thread starter Les serres de Paolo Inc.
- Start date
Les serres de Paolo Inc.
Carnivorous Plant Addict
On observation, hoping no infections…..His relatives watching him during the nights (the 2 guys on the moving tray
)
King Karnivores
Carnivorous Plant Addict
Im curious on how this guy did. I know putting nepenthes into TC from meristem tissue is notoriously hard. Did this guy survive?
Les serres de Paolo Inc.
Carnivorous Plant Addict
The samples didn’t survive because of contamination. I didn’t remove enough layers to be close or to get to the meristem. But at least the plant gave me a basal shoot so in a way it survived.
Today I did my first meristem attempt on a Nepenthes…it’s so hard lolllll But I think I got it, will see in a few weeks. Much easier with seeds
TC is so addictive and so fun, I have been going full trottle but now realize I have so many successfull reproduction that I have to think to built a third Greenhouse to support the quantity of production, it’s just too insane…and my second Greenhouse will be ready in August but will be full by end of year lollll
King Karnivores
Carnivorous Plant Addict
TC is definitely fun once you get a hold of contam. My first few batches had tons of contam but I think I've finally got good enough at sterilization to have good results.The samples didn’t survive because of contamination. I didn’t remove enough layers to be close or to get to the meristem. But at least the plant gave me a basal shoot so in a way it survived.
Today I did my first meristem attempt on a Nepenthes…it’s so hard lolllll But I think I got it, will see in a few weeks. Much easier with seeds
TC is so addictive and so fun, I have been going full trottle but now realize I have so many successfull reproduction that I have to think to built a third Greenhouse to support the quantity of production, it’s just too insane…and my second Greenhouse will be ready in August but will be full by end of year lollll
What sterilization protocol do you use for meristem? I'd like to give it a try
Les serres de Paolo Inc.
Carnivorous Plant Addict
Yes at first contamination is the number one ennemy. Now my problem is space to acclimate all theses plants lolllllllllllll
For meristem, normal protocol like use of bleach for the explant, then when you remove all the layers, make sure you sterilize your instrument while pealing off the layer because when you reach the meristem it’s already free of contamination. It’s so small that you will need magnifier lens to manipulate it.