Pollen collection

Lloyd Gordon

Cactus micrografter newbie.
Staff member
I may have a variant of the usual method.
I took a fine forceps and pulled* off an anther taking the green filament with it. Maneuver the filament in the forceps so you're holding it just above the cut end. You hold it so the loose end of the anther is up and the other end is just above the wax paper. Then touch a 512 Hz tuning fork to the loose end of the anther and the pollen pours out. Not too high or it blows away.
Repeat 16 times for all the anthers. Keep sealed in freezer.
I found it much easier. No more fussing with odd positions or holding the wax paper in your third hand. No wasted pollen. Worked great! The "T" is where you touch the tuning fork.
Be careful pulling off the anther, you don't want to rip off part of the ovary!
About 4 days after flower opening, I test one anther a day-use the technique above just over a black surface (I use my keyboard). If you get a little flurry of pollen, harvest. Otherwise repeat next day.
*Actually, "snip" it off with the forceps by squeezing. You don't want to rip the ovary wall as this aborts the developing seeds. If you pull the anther of the filament, you can hold the wide end of the anther up and the small end down-touch the tuning fork to the forceps tip multiple times to get all the pollen. Collecting into tiny ziploc bags is very convenient.
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Today my second flower was ready to pollinate. I tried something new again. I just took the flower and gently placed the stigma onto the waxed paper to carefully deposit some pollen on it. Seemed to work well. No need for brushes or toothpicks. The pollen seemed to hold to the wax paper so it didn't slip away.
 
"In terms of plants I want... I absolutely do not need any more plants whatsoever! I am full up and have too many to deal with as it is.'

So much for New Years Resolutions :p

Very interesting. Hope I can get my Nep. Ventricosa to flower in years to come
get ready to off load them D Hams and U Gibba Lloyd :p CONNOR AT YOUR :D
 
Very cool, Lloyd. I’m waiting on a Heliamphora seed pod to ripen right now. What kind of Heliamphora is flowering for you?
 
I just checked (lights are on at night to reduce power costs - thanks, Lloyd!) and I have 4 new plants sending up flower stalks. This information has come just in time!

Avery - Could we talk pollen swaps sometime?
 
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Note the anthers have turned from a light yellow and a plump texture to a deeper almost brown yellow and are a little less plump. I've been trying an anther a night and tonight was the first time I got some pollen. I'm going to try again tomorrow. Before when the anthers were brown and thinner, the pollen really flowed out. I'm going to try to figure out the optimum anther qualities.
 
Looking good Lloyd!

Most of my flowers tend to not open fully, they go red, and seem a little 'dry' to me...

Would you share a photo of the plant? :D
 
I'm not convinced my clone is particularly different or good. I just think that I accidentally created the perfect environment for it and just as accidentally introduced it.
 
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