Big decision mid January for in-vitro program, thanks for your input (CLOSED)

Which species you would like to have new variety (new creation thru mutation) max 3 choices

  • Drosera

    Votes: 7 29.2%

  • Cephalotus

    Votes: 6 25.0%

  • Dionaea muscipula

    Votes: 7 29.2%

  • Utricularia

    Votes: 4 16.7%

  • Nepenthes

    Votes: 8 33.3%

  • Pinguicula

    Votes: 10 41.7%

  • Sarracenia

    Votes: 10 41.7%

  • Heliamphora

    Votes: 4 16.7%
  • Total voters
    24
  • Poll closed .
Curious - are you considering developing plants for harsher climates, or for beginners?
 
Les serres de Paolo Inc. said:
Thanks, yes it’s kind of fun and exciting in the same time! There’s the waiting time to see if the culture will produce multiplication and always cool to see strange things growing like the callus.

It took me around 18 months to gather around the equipment. I have learned by trial and error, now my contamination is less than 5% and trying to reach less than 1%

Here’s some picture I took while doing some in-vitro Today

Bleaching and rinsing operation of the plants parts.

View attachment 26574View attachment 26575View attachment 26576
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What protocol do you use for sterilizing explants? I've tried getting Cephalotus into TC from leaf pullings without luck :/
 
KingLouisCLXXII said:
What protocol do you use for sterilizing explants? I've tried getting Cephalotus into TC from leaf pullings without luck :/
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For sterilizing explants I simply use bleach, sterilized water and 2 drops of soap and soak about 5 min to 8 min depending of the fragility of the explant. The bleaching solution as to be stirred during all the process so the explant moves into the solution instead of floating. Then the explant goes to rinsing with sterilized water which I put some drops of hydrogen peroxyde 29%. Just before putting the explant into the culture vessel I put them for 2 sec in 70% Ethanol then quickly rinse with sterilized water and then in the culture vessel.

I dind’t yet tried with Ceph leaf because I don’t have enough leaf yet but I have tried with closed pitcher, I have one that is on trial and waiting to see if it works. The easier way would be with seeds but will not necessarily be as true as the plant. Will wait this spring for new leaf formation to do the test.
 
Les serres de Paolo Inc. said:
For sterilizing explants I simply use bleach, sterilized water and 2 drops of soap and soak about 5 min to 8 min depending of the fragility of the explant. The bleaching solution as to be stirred during all the process so the explant moves into the solution instead of floating. Then the explant goes to rinsing with sterilized water which I put some drops of hydrogen peroxyde 29%. Just before putting the explant into the culture vessel I put them for 2 sec in 70% Ethanol then quickly rinse with sterilized water and then in the culture vessel.

I dind’t yet tried with Ceph leaf because I don’t have enough leaf yet but I have tried with closed pitcher, I have one that is on trial and waiting to see if it works. The easier way would be with seeds but will not necessarily be as true as the plant. Will wait this spring for new leaf formation to do the test.
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What % bleach do you use?
 
Thanks for all the input!

3 hours meeting and I’m so excited with the project!

I’m closing this survey as it gives me a good idea. So much work to be done this year and will keep you inform in a new post for the in-vitro progress that will be update with time.

Thank you, this group is awesome! So much help is offered and really appreciate it!

Cheers

Paolo
 
Only issue in the business model us that carnivorous plants appeal is very small to plant enthusiasts. For some unknown reason, carnivorous plants appeal more to men. At pLant stores, i only see maybe 3 -6 kinds at best with the vft first, sarrencia second , then a some cap dew plants and rare occasion, a ping...women plant lovers collectoRs go for stuff with tropical leaves, hoyas, cacti, succulents etc.
 
jackie quan said:
Only issue in the business model us that carnivorous plants appeal is very small to plant enthusiasts. For some unknown reason, carnivorous plants appeal more to men. At pLant stores, i only see maybe 3 -6 kinds at best with the vft first, sarrencia second , then a some cap dew plants and rare occasion, a ping...women plant lovers collectoRs go for stuff with tropical leaves, hoyas, cacti, succulents etc.
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That’s because the carnivorous plants were not produce for variety but for only the most know and popular. I’m changing this in Province of Quebec, supplying a thousand carnivorous plants per month thru high season. In the next years, people will have more and more choices like Cephalotus, Heliamphora, Nepenthes, Sarracenia etc Most important would be the quantity available for large mass and price will go down so more people can afford it. This year I will supply more plants than last year and my delivery location will increase as I will enlarge my delivery zones.

Here are my new culture vessel, with one thousand I should be good for the next 3 months :p

26640
 
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jackie quan said:
Only issue in the business model us that carnivorous plants appeal is very small to plant enthusiasts. For some unknown reason, carnivorous plants appeal more to men. At pLant stores, i only see maybe 3 -6 kinds at best with the vft first, sarrencia second , then a some cap dew plants and rare occasion, a ping...women plant lovers collectoRs go for stuff with tropical leaves, hoyas, cacti, succulents etc.
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I'm not a woman so can't say for certain, but after showing some nepenthes to my wife, I think women just have different priorities, color and beauty for example (e.g peristome/pitcher color, shape), while a lot of men are into weird things like "teeth" or hunch back of aristolchioides or klossii.

Several times I show my wife pictures of eddys, hamata, villosa, or klossi, and she wasn't show much reaction at all. But things like burb, vent alba "porcelain", for example, captivated her.

Not only that, most average houseplants collectors want something that is not too difficult to grow, can be used as house decorations, and not super expensive, which is why I think foliage plants are much more in demand.
 
Les serres de Paolo Inc. said:
That’s because the carnivorous plants were not produce for variety but for only the most know and popular. I’m changing this in Province of Quebec, supplying a thousand carnivorous plants per month thru high season. In the next years, people will have more and more choices like Cephalotus, Heliamphora, Nepenthes, Sarracenia etc Most important would be the quantity available for large mass and price will go down so more people can afford it. This year I will supply more plants than last year and my delivery location will increase as I will enlarge my delivery zones.

Here are my new culture vessel, with one thousand I should be good for the next 3 months :p

View attachment 26640
Click to expand...

I'm so looking forward to the day you become the wistuba or BCP of Canada, the day I no longer need to wait for a group order to afford rare, exotic nepenthes :cool:
 
Except for the obvious, woman/male differences are mostly culture based, including choice of Nepenthes.
 
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A like statistics so here’s one of my add on Facebook and the ratio women vs man, you can also see the age category.

The add reached 70.5% women vs 29.5% men

At my greenhouse boutique I have more women than men buying carnivorous plants. When someone needs a special plant or looking for something rare it’s more men than women that ask for that.


26659
 
Les serres de Paolo Inc. said:
A like statistics so here’s one of my add on Facebook and the ratio women vs man, you can also see the age category.

The add reached 70.5% women vs 29.5% men

At my greenhouse boutique I have more women than men buying carnivorous plants. When someone needs a special plant or looking for something rare it’s more men than women that ask for that.


View attachment 26659
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Interesting.
 
Interestign the term"husbandry" is used as opposed to "wifery" for plant cultivation. For my corals hobbies, commonly appealing much more to men, husbandry term is used a lot.
 
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