I'm going to put some Sarr's in TC. I don't want to stratify them as they will likely soak up some bacteria over that time period. Should I presoak them in GA3 (concentration?) for 24 hours or add some GA3 to medium (concentration?).?
You can soak the seeds in GA3, and then sterilize them.I'm going to put some Sarr's in TC. I don't want to stratify them as they will likely soak up some bacteria over that time period. Should I presoak them in GA3 (concentration?) for 24 hours or add some GA3 to medium (concentration?).?
Was that grown from the batch of seeds Kdawg sent out?Darlingtonia
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Thanks for the advice. I'll pressure cook the plates when I'm done with them. I don't plan on getting plants out of this, it was really just a test.You put the agar in after pH'ing and before autoclaving. I put the agar in each container separately just before autoclaving.
As an amateur, I would not use antibiotics. The issue of creating superbugs is scary. Great care should be used, including incineration of contaminated medium and autoclaving contaminated supplies.
In my experience, contamination is ~ 100% mould. If you're getting bacterial contamination, you need to discover what's wrong with your technique. The antibiotics have to be properly disposed of.
In general, contamination means imperfect technique. Everybody gets the occasional mold but you can keep it to a minimum.
Here's an idea how I prepare mine: http://www.carnivorousplantsociety.ca/index.php?threads/tc-guide-preparing-the-media.392/Do you autoclave your agar? I poured some plates yesterday and I just used a pressure cooker. I'm not super confident with my sterile culture so I added some Kanamycin monosulfate. I was going to use some falcon tubes instead of plates but I found out the hard way that they weren't autoclaveable.
It used to be 100mlWhat's the volume of the medium?
they don't look like they have developed pitchers. is it because they get all they need from the soil and therefore don't need extra source of food?Nepenthes bellii
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That's correct, many Nepenthes species in TC don't spend much effort working on their pitchers because they get the nutrients needed directly from the jelly.they don't look like they have developed pitchers. is it because they get all they need from the soil and therefore don't need extra source of food?
WOW! Do you take these guys out at all when fully mature?Nepenthes hispida
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Nepenthes glabrata
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Nepenthes x hookeriana
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Yes, I take them out once in a while.WOW! Do you take these guys out at all when fully mature?