Plant Growth Regulators (PGR) Reference

[WillyCKH]

Plant Growth Regulators (PGR) Reference
Here are some useful references borrowed from Sigma-Aldrich.

Preparation and Use
To prepare a 1 mg/ml stock solution:

1. Add 100 mg of the plant growth regulator to a 100 ml volumetric flask or other glass container.
2. Add 2-5 ml of solvent to dissolve the powder.
3. Once completely dissolved, bring to volume with double processed water.
4. Stirring the solution while adding water may be required to keep the material in solution.
5. Store the stock solution as recommended in the tables.
6. Add 1.0 ml of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/L of the plant growth regulator in the culture medium.

Auxins
Plant Cell Culture Tested Auxins are generally used in plant cell culture at a concentration range of 0.01-10.0 mg/L. When added in appropriate concentrations, they may regulate cell elongation, tissue swelling, cell division, formation of adventitious roots, inhibition of adventitious and axillary shoot formation, callus initiation and growth, and induction of embryogenesis.

Product Name	Mol. Wt.	µM for 1mg/L	Solvent	Diluent	Powder Storage	Liquid Storage	Steriliz-	Working Conc. (mg/L)
p-Chlorophenoxyacetic acid (4-CPA)	186.6	5.36	EtOH	—	Room Temperature	2-8°C	CA	0.1-10.0
2,4-Dichlorophenoxyacetic acid	221	4.53	—	—	Room Temperature	2-8°C	CA	0.01-6.0
2,4-Dichlorophenoxyacetic acid Sodium salt	243	4.12	Water	—	Room Temperature	2-8°C	CA	0.01-6.0
Indole-3-acetic acid Free acid (IAA)	175.2	5.71	EtOH/1N NaOH	Water	-0°C	-0°C	CA/F	0.01-3.0
Indole-3-acetic acid Sodium salt	197.2	5.07	Water	Water	2-8°C	-0°C	CA/F	0.01-3.0
Indole-3-acetic acid methyl ester	189.2	5.29	—	—	2-8°C	2-8°C	—	—
Indole-3-acetyl-L-aspart acid	290.3	3.45	0.5N NaOH	Water	-0°C	-0°C	F	0.01-5.0
Indole-3-butyric acid (IBA)	203.2	4.9	EtOH/1N NaOH	Water	2-8°C	-0°C	CA/F	0.1-10.0
Indole-3-butyric acid Potassium salt (K-IBA)	241.3	4.14	Water	—	2-8°C	-0°C	CA/F	0.1-10.0
alpha-Naphthaleneacetic acid Free acid (NAA)	186.2	5.37	1N NaOH	Water	Room Temperature	2-8°C	CA	0.1-10.0
beta-Naphthoxyacetic acid Free acid (NOA)	202.2	4.95	1N NaOH	Water	Room Temperature	2-8°C	CA	0.1-10.0
Phenylacetic acid (PAA)	136.2	7.34	EtOH	—	Room Temperature	2-8°C	CA/F	0.1-50.0
Picloram	241.5	4.14	DMSO	—	Room Temperature	2-8°C	CA	0.01-10.0
2,4,5-Trichlorophenoxyacetic acid (2,4,5-T)	255.5	3.91	EtOH	—	Room Temperature	2-8°C	CA	0.01-5.0
2,3,5-Triiodobenzoic acid Free acid (TIBA)	499.8	2	1N NaOH	Water	-0°C	-0°C	F	0.05-5.0

Cytokinins
Plant Cell Culture Tested Cytokinins are generally used in plant cell culture at a concentration range of 0.1-10.0 mg/L. When added in appropriate concentrations, they may regulate cell division, stimulate axillary and adventitious shoot proliferation, regulate differentiation, inhibit root formation, activate RNA synthesis and stimulate protein and enzyme activity.

Product Name	Mol. Wt.	µM for 1mg/L	Solvent	Diluent	Powder Storage	Liquid Storage	Steriliz-	Working Conc. (mg/L)
Adenine Free base	135.1	7.4	1.0 HCl	Water	Room Temperature	2-8°C	CA	50-250
Adenine hemisulfate Hemisulfate salt	184.2	5.43	Water	—	Room Temperature	2-8°C	CA	50-250
6-Benzylaminopurine (BA)	225.3	4.44	1N NaOH	Water	Room Temperature	2-8°C	CA/F	0.1-5.0
6-Benzylaminopurine Hydrochloride	261.7	3.82	Water	—	Room Temperature	2-8°C	CA/F	0.1-5.0
N-Benzyl-9-(2-tetrahydropyranyl)adenine (BPA)	309.4	3.23	EtOH	—	-0°C	-0°C	CA/F	0.1-5.0
N-(2-Chloro-4-pyridyl)-N'-phenylurea (4-CPPU)	247.7	4.04	DMSO	—	2-8°C	2-8°C	F	0.001-1.0
6-(gamma,gamma-Dimethylallylamino)purine (2iP)	203.2	4.92	1N NaOH	Water	-0°C	-0°C	CA/F	1.0-30.0
1,3-Diphenylurea (DPU)	212.3	4.71	DMSO	—	Room Temperature	2-8°C	F	0.1-1.0
Kinetin	215.2	4.65	1N NaOH	Water	-0°C	-0°C	CA/F	0.1-5.0
Kinetin Hydrochloride	251.7	3.97	Water	—	-0°C	-0°C	CA/F	0.1-5.0
1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea	220.2	4.54	DMSO	—	Room Temperature	2-8°C	CA/F	0.001-0.05
trans-Zeatin Free base	219.2	4.56	1N NaOH	Water	-0°C	-0°C	CA/F	0.01-5.0
Zeatin	219.2	4.56	1N NaOH	Water	-0°C	-0°C	CA/F	0.01-5.0
trans-Zeatin Hydrochloride	255.7	3.91	Water	—	-0°C	-0°C	CA/F	0.01-5.0
trans-Zeatin riboside	351.4	2.85	1N NaOH	Water	-0°C	-0°C	F	0.01-5.0

Miscellaneous Plant Growth Regulators
These PGRs are less commonly used in Plant Cell Culture.

Product Name	Mol. Wt.	µM for 1mg/L	Solvent	Diluent	Powder Storage	Liquid Storage	Steriliz-	Working Conc. (mg/L)
(±)-cis,trans-Abscisic acid (ABA)	264.3	3.78	1N NaOH	Water	-0°C	-0°C	CA/F	0.1-10.0
Ancymidol	256.3	3.9	DMSO	—	2-8°C	-0°C	CA/F	1.0-10.0
Chlorocholine chloride (CCC)	158.1	6.33	Water	—	Room Temperature	2-8°C	F	up to 500
3,6-Dichloro-o-anisic acid (Dicamba)	221	4.52	EtOH/Water	—	2-8°C	2-8°C	F	0.01-10.0
Gibberellic acid (GA3)	346.4	2.89	EtOH	—	Room Temperature	2-8°C	CA/F	0.01-5.0
Gibberellic acid Potassium salt (K-GA3)	384.5	2.6	Water	—	2-8°C	-0°C	CA/F	0.01-5.0
Gibberellin A4 Free acid (GA4)	332.4	3.01	EtOH	—	-0°C	-0°C	F	0.01-5.0
(±)-Jasmonic acid	210.3	4.76	EtOH	—	2-8°C	-0°C	F	0.01-100.0
Phloroglucinol	126.1	7.93	Water	—	Room Temperature	2-8°C	CA/F	up to 162
N-(Phosphonomethyl)glycine (Glyphosate)	169.1	5.91	1N NaOH	Water	Room Temperature	2-8°C	F	—
Succinic acid 2,2-dimethylhydrazide	160.2	6.24	Water	—	2-8°C	2-8°C	CA/F	0.1-10.0

CA = coautoclavable with other media components.
F = filter sterilize.
CA/F = coautoclavable with other media components, however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be filter sterilized.

 

[Viridis]

It should be noted 1N NaOH= 40g/L

 

[Lloyd Gordon]

Be careful with acids and and alkalis.

 

[WillyCKH]

Definitely need to be careful around PGR, acids and alkalis because these chemical can be dangerous and harmful! Make sure to wear gloves, mask and safety goggles, at the very least.

 

[Viridis]

Definitely need to be careful around PGR, acids and alkalis because these chemical can be dangerous and harmful! Make sure to wear gloves, mask and safety goggles, at the very least.

And work in a well ventilated area when using/mixing them!

 

[John Yates]

KOH is my referred method of dissolving the PGR's that is use , but is probably the most dangerous as well ,

Nicley done Will , great scale ,great for a easy reference , thank you

 

[WillyCKH]

Thank you, John

 

[Lloyd Gordon]

My cacti seedlings were going a bit rogue in TC with no PGR's. So I replated with a low concentration of Meta-topolin thinking it should normalize them. Bad idea! They went crazy with multiple growth points, increasing callous and weird lumpy growths on roots. Just replated them again without any PGR's.

 

[John Yates]

yes has happened to me in neps too Lloyd , lower your sugar , and media strenth to combat it , callus from my side of things is when its to strong,your plants are all ready excitable , ,just lower the excitement

 

[carniferryplant]

I never did tissue culture and it seems very complicated.I think I just have to get some nice agar and plant some plants in the gel.

 

[Lloyd Gordon]

I guess you mean in a sterile fashion? Agar is a great medium for microbes.