Plant Growth Regulators (PGR) Reference

WillyCKH

CPSC Moderator
Staff member
Plant Growth Regulators (PGR) Reference
Here are some useful references borrowed from Sigma-Aldrich.

Preparation and Use
To prepare a 1 mg/ml stock solution:
  1. Add 100 mg of the plant growth regulator to a 100 ml volumetric flask or other glass container.
  2. Add 2-5 ml of solvent to dissolve the powder.
  3. Once completely dissolved, bring to volume with double processed water.
  4. Stirring the solution while adding water may be required to keep the material in solution.
  5. Store the stock solution as recommended in the tables.
  6. Add 1.0 ml of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/L of the plant growth regulator in the culture medium.
Auxins
Plant Cell Culture Tested Auxins
are generally used in plant cell culture at a concentration range of 0.01-10.0 mg/L. When added in appropriate concentrations, they may regulate cell elongation, tissue swelling, cell division, formation of adventitious roots, inhibition of adventitious and axillary shoot formation, callus initiation and growth, and induction of embryogenesis.

Product NameMol. Wt.µM for 1mg/LSolventDiluentPowder StorageLiquid StorageSteriliz-Working Conc. (mg/L)
p-Chlorophenoxyacetic acid (4-CPA)186.65.36EtOHRoom Temperature2-8°CCA0.1-10.0
2,4-Dichlorophenoxyacetic acid2214.53Room Temperature2-8°CCA0.01-6.0
2,4-Dichlorophenoxyacetic acid Sodium salt2434.12WaterRoom Temperature2-8°CCA0.01-6.0
Indole-3-acetic acid Free acid (IAA)175.25.71EtOH/1N NaOHWater-0°C-0°CCA/F0.01-3.0
Indole-3-acetic acid Sodium salt197.25.07WaterWater2-8°C-0°CCA/F0.01-3.0
Indole-3-acetic acid methyl ester189.25.292-8°C2-8°C
Indole-3-acetyl-L-aspaRoom Temperatureic acid290.33.450.5N NaOHWater-0°C-0°CF0.01-5.0
Indole-3-butyric acid (IBA)203.24.9EtOH/1N NaOHWater2-8°C-0°CCA/F0.1-10.0
Indole-3-butyric acid Potassium salt (K-IBA)241.34.14Water2-8°C-0°CCA/F0.1-10.0
alpha-Naphthaleneacetic acid Free acid (NAA)186.25.371N NaOHWaterRoom Temperature2-8°CCA0.1-10.0
beta-Naphthoxyacetic acid Free acid (NOA)202.24.951N NaOHWaterRoom Temperature2-8°CCA0.1-10.0
Phenylacetic acid (PAA)136.27.34EtOHRoom Temperature2-8°CCA/F0.1-50.0
Picloram241.54.14DMSORoom Temperature2-8°CCA0.01-10.0
2,4,5-Trichlorophenoxyacetic acid (2,4,5-T)255.53.91EtOHRoom Temperature2-8°CCA0.01-5.0
2,3,5-Triiodobenzoic acid Free acid (TIBA)499.821N NaOHWater-0°C-0°CF0.05-5.0

Cytokinins
Plant Cell Culture Tested Cytokinins
are generally used in plant cell culture at a concentration range of 0.1-10.0 mg/L. When added in appropriate concentrations, they may regulate cell division, stimulate axillary and adventitious shoot proliferation, regulate differentiation, inhibit root formation, activate RNA synthesis and stimulate protein and enzyme activity.

Product NameMol. Wt.µM for 1mg/LSolventDiluentPowder StorageLiquid StorageSteriliz-Working Conc. (mg/L)
Adenine Free base135.17.41.0 HClWaterRoom Temperature2-8°CCA50-250
Adenine hemisulfate Hemisulfate salt184.25.43WaterRoom Temperature2-8°CCA50-250
6-Benzylaminopurine (BA)225.34.441N NaOHWaterRoom Temperature2-8°CCA/F0.1-5.0
6-Benzylaminopurine Hydrochloride261.73.82WaterRoom Temperature2-8°CCA/F0.1-5.0
N-Benzyl-9-(2-tetrahydropyranyl)adenine (BPA)309.43.23EtOH-0°C-0°CCA/F0.1-5.0
N-(2-Chloro-4-pyridyl)-N'-phenylurea (4-CPPU)247.74.04DMSO2-8°C2-8°CF0.001-1.0
6-(gamma,gamma-Dimethylallylamino)purine (2iP)203.24.921N NaOHWater-0°C-0°CCA/F1.0-30.0
1,3-Diphenylurea (DPU)212.34.71DMSORoom Temperature2-8°CF0.1-1.0
Kinetin215.24.651N NaOHWater-0°C-0°CCA/F0.1-5.0
Kinetin Hydrochloride251.73.97Water-0°C-0°CCA/F0.1-5.0
1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea220.24.54DMSORoom Temperature2-8°CCA/F0.001-0.05
trans-Zeatin Free base219.24.561N NaOHWater-0°C-0°CCA/F0.01-5.0
Zeatin219.24.561N NaOHWater-0°C-0°CCA/F0.01-5.0
trans-Zeatin Hydrochloride255.73.91Water-0°C-0°CCA/F0.01-5.0
trans-Zeatin riboside351.42.851N NaOHWater-0°C-0°CF0.01-5.0

Miscellaneous Plant Growth Regulators
These PGRs are less commonly used in Plant Cell Culture.

Product NameMol. Wt.µM for 1mg/LSolventDiluentPowder StorageLiquid StorageSteriliz-Working Conc. (mg/L)
(±)-cis,trans-Abscisic acid (ABA)264.33.781N NaOHWater-0°C-0°CCA/F0.1-10.0
Ancymidol256.33.9DMSO2-8°C-0°CCA/F1.0-10.0
Chlorocholine chloride (CCC)158.16.33WaterRoom Temperature2-8°CFup to 500
3,6-Dichloro-o-anisic acid (Dicamba)2214.52EtOH/Water2-8°C2-8°CF0.01-10.0
Gibberellic acid (GA3)346.42.89EtOHRoom Temperature2-8°CCA/F0.01-5.0
Gibberellic acid Potassium salt (K-GA3)384.52.6Water2-8°C-0°CCA/F0.01-5.0
Gibberellin A4 Free acid (GA4)332.43.01EtOH-0°C-0°CF0.01-5.0
(±)-Jasmonic acid210.34.76EtOH2-8°C-0°CF0.01-100.0
Phloroglucinol126.17.93WaterRoom Temperature2-8°CCA/Fup to 162
N-(Phosphonomethyl)glycine (Glyphosate)169.15.911N NaOHWaterRoom Temperature2-8°CF
Succinic acid 2,2-dimethylhydrazide160.26.24Water2-8°C2-8°CCA/F0.1-10.0

CA = coautoclavable with other media components.
F = filter sterilize.
CA/F = coautoclavable with other media components, however, some loss of activity may occur. This can be compensated for by increasing component concentration. Component may be filter sterilized.
 

WillyCKH

CPSC Moderator
Staff member
Definitely need to be careful around PGR, acids and alkalis because these chemical can be dangerous and harmful! Make sure to wear gloves, mask and safety goggles, at the very least.
 
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