Glass bead sterilizer

Lloyd Gordon

Parasitic Plant Aficionado
Staff member

I finally got sick of burning my fingers and dropping flaming alcohol on the flow hood and carpet. I was afraid I'd have a bad fire eventually. I bought the small one.
 

pinhead

Sprout
Thats what I have.
Been using it for a couple of years without any problem.

I still dip the tools in alcohol when I remove it from the sterilizer. Alcohol evaporates immediately because the tools can become quite hot.

That is still safer than flaming the alcohol off in an open flame.
 

Viridis

Seedling
Stupid question—Where are you putting the cord? Are you drilling a hole in the side of the hood, passing it through then sealing it? Or is it just coming back out the front where you're sitting?

I've been considering getting one for a while (I may have lit my workspace on fire once or twice).
 

pinhead

Sprout
I have the sterilzer sitting outside of my laminar flow hood. The tools are hot enough that when you take them out of the sterilizer the convection currents shouldn't allow any spores land on the tools. I tried to put the sterilzer in the hood with the cord coming out the front, but I don't think it makes a difference.

I took a few Microbiology courses in university and we didn't worry about contaminating the inoculation loops after we flamed them.
 

Lloyd Gordon

Parasitic Plant Aficionado
Staff member
Based on the above, I'll try it just outside and then inside if I have a problem. 300°C should be good for a few seconds and cm's.
 

DennisZ

Carnivore
I have the sterilzer sitting outside of my laminar flow hood. The tools are hot enough that when you take them out of the sterilizer the convection currents shouldn't allow any spores land on the tools. I tried to put the sterilzer in the hood with the cord coming out the front, but I don't think it makes a difference.

I took a few Microbiology courses in university and we didn't worry about contaminating the inoculation loops after we flamed them.
Thanks for the info! It's good to know since my LFH (wonderfully crafted by Willy) gets a bit tight on space with the sterilizer in.
 

Lloyd Gordon

Parasitic Plant Aficionado
Staff member
I use my sterilizer inside the hood at the front edge, the cord just loops around. So far my contamination rate has gone way down (knock on wood). I highly recommend glass bead sterilizers. I set it to 300°C. The handle is not hot to hold. I stick the forceps into the agar to cool it (the agar hisses as it boils for a second).
 

WillyCKH

CPSC Moderator
Staff member
It works for me too! I just wish the sterilizer is deeper so I can use it for longer tools.
 
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