Hi
@Zoman, you will want to add Plant growth regulators to induce callus. You can start with BAP. By the way, you can refer to the article you shared in the
Induction of shoots and roots section.
D. capensis multiplies steadily in TC, I am not sure what you are trying to do, is this just for practice?
If you have more questions please post a new thread or send me a private message.
Cheers,
Willy